Butanol Productionwith High Yield and Carbon Conversion Using Novel Biocatalysts
2016 USDA-NIFA Integrated Award
Principal Investigators and affiliations:
PI: Dr. Hasan Atiyeh (Oklahoma State University)
Co-PI: Dr. Thaddeus Ezeji (Ohio State University)
Start Date: 9/01/2016
End Date: 8/31/2018
The overall goal of this project is to increase butanol yield by more than 30% producing up to 40 g/L butanol, or 90 g/L total ABE, and improve the economics of production via the integrated novel conversion process and utilization of lignocellulosic biomass and CO2 (greenhouse gas, GHG).
- Develop butanol fermentation in two-stage reactors with and without in situ ABE recovery.
- Investigate butanol fermentation with the co-culture in a single bioreactor with and without in situ ABE recovery.
- Integrate LDMICs tolerance genes (LTG) into the chromosome of Cb to increase catalytic proficiency of lignocellulosic biomass conversion to butanol.
Successful completion of this project will involve development of a novel biocatalytic process with increased conversion efficiency to make biofuels and biobased products. At the conclusion of this project, the factors that allow both microorganisms to thrive in a co-culture environment and the reactor operating parameters that result in maximum butanol productivity and yield will be identified. A process for butanol production from lignocellulosic biomass with high yield and carbon conversion efficiencies compared to current technologies will be developed.